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'''Gut-brain signalling''' describes the interaction between the [[gastrointestinal tract]] and the brain, and how secretion of varying [[hormone]]s from different areas of the body causes [[appetite]]-enhancing and [[satiety]] signals to be sent to the brain.  The hormones that have been most intensely studied are: [[ghrelin]], [[obestatin]], [[cholecystokinin]] (CCK), [[GLP-1]], [[peptide YY]] (PYY) and [[insulin]] which all play major roles in appetite regulation.  The [[vagus nerve]] is also a key mediator of regulation, and all of these inputs are processed by areas in the brain such as the [[hypothalamus]] and the [[nucleus tractus solitarii]] (NTS).
'''Gut-brain signalling''' describes the interaction between the [[gastrointestinal tract]] and the brain, and how secretion of varying [[hormone]]s from different areas of the body causes [[appetite]]-enhancing and [[satiety]] signals to be sent to the brain.  The hormones that have been most intensely studied are: [[ghrelin]], [[obestatin]], [[cholecystokinin]] (CCK), [[glucagon-like peptide-1]] (GLP-1), [[peptide YY]] (PYY) and [[insulin]] which all have roles in appetite regulation.  The [[vagus nerve]] is also a key mediator of regulation, and all of these inputs are processed by areas in the brain such as the [[hypothalamus]] and the [[nucleus tractus solitarii]] (NTS).


==Anorexic Signals==
==Anorexic Signals==
{{Image|diagram 3.jpg|right|500px|''Gut-Brain signaling Pathways'' Proteins and hormones activate brain pathways in different ways, either by eventual vagal activation or through peripheral circulation. The Nucleus Tractus Solitarus and the arcuate nucleus are then activated. }}
{{Image|diagram 3.jpg|right|500px|''Gut-Brain signaling Pathways'' Proteins and [[hormone]]s activate brain pathways in different ways, either by eventual vagal activation or through peripheral circulation. The [[nucleus tractus solitarii]] and the [[arcuate nucleus]] are then activated. }}
'''[[Cholecystokinin]]''' (CCK) is a peptide hormone synthesised  by L-cells in the mucosal epithelium of the [[duodenum]], and secreted in response to the presence of partially digested [[lipid]]s and [[protein]]s. CCK inhibits gastric emptying and stimulates the release of digestive enzymes from the [[pancreas]] and [[bile]] from the [[gall bladder]] by acting at CCK-A receptors (mainly found in the periphery but also found in some areas of the CNS). Because gastric emptying is inhibited, the partially digested lipids and proteins are exposed to the digestive enzymes and bile so are further broken down. As the lipids and proteins are broken down, CCK secretion declines.  
'''[[Cholecystokinin]]''' (CCK) is a hormone synthesised  by [[L-cells]] in the mucosal epithelium of the [[duodenum]], and secreted in response to the presence of partly digested [[lipid]]s and [[protein]]s. CCK inhibits gastric emptying and stimulates the release of digestive enzymes from the [[pancreas]] and [[bile]] from the [[gall bladder]] by acting at CCK-A receptors on afferent vagal nerve endings in the gastrointestinal tract. Because gastric emptying is inhibited, the partly digested lipids and proteins are exposed to the digestive enzymes and bile so are further broken down. As the lipids and proteins are broken down, CCK secretion declines.  


CCK acts as a ‘gatekeeper’ for the response of other gut-brain signalling hormones on the afferent vagal neurons. At low levels (after fasting), CCK stimulates the expression of receptors associated with the stimulation of food intake, including receptors for [[melanin concentrating hormone]] (MCH)-1 and [[cannabinoid] CB1 receptors. At high levels (after food consumption), MCH-1 and CB1 receptors are down regulated. Therefore CCK present at a high or low concentration can affect how afferent vagal neurons respond to other neurohormones.
CCK is a ‘gatekeeper’ for the response of other gut-brain signalling hormones on the afferent vagal neurons. At low levels (after fasting), CCK stimulates the expression of receptors associated with the stimulation of food intake, including receptors for [[melanin concentrating hormone]] (MCH)-1 and [[cannabinoid]] CB1 receptors. At high levels (after food consumption), MCH-1 and CB1 receptors are down-regulated. Therefore CCK, at a high or low concentration, can affect how afferent vagal neurons respond to other neurohormones.


In rats, CCK inhibits food intake in younger individuals more effectively than in older individuals. It also has a greater effect in males than in females.
'''Glucagon-like peptide-1''' (GLP-1) is a hormone secreted from L-cells in the mucosal epithelium of the duodenum and small intestine. It is derived from the ''pro-glucagon'' gene, and is secreted into the circulation in response to the presence of nutrients. It acts at the [[pancreas]], where it stimulates [[insulin]] secretion and suppresses [[glucagon]] secretion; it also increases insulin sensitivity. GLP-1 also activates anorexigenic neurons in the [[arcuate nucleus]] via the caudal brainstem, thereby suppressing [[appetite]]. It also decreases gastric emptying, so adds to the feeling of being ‘full’. At higher concentrations, GLP-1 causes [[nausea]], and can induce [[conditioned taste aversion]], where the brain associates the taste of a certain food with being toxic (usually after an individual consumes a food that had made them sick).


'''Glucagon-like peptide-1''' (GLP-1) is a hormone secreted from L-cells in the mucosal epithelium of the duodenum and small intestine. It is derived from the ''pro-glucagon'' gene, and is secreted into the circulation in response to the presence of nutrients. It acts at the [[pancreas]], where it stimulates [[insulin]] secretion and suppresses [[glucagon]] secretion. It also increases insulin sensitivity. GLP-1 also acts on inhibitory neurons in the [[arcuate nucleus]] via the caudal brainstem. Activation of these inhibitory neurons induces satiety and decreases food intake/hunger. It also decreases gastric emptying so adds to the feeling of being ‘full’. At higher concentrations, GLP-1 causes [[nausea]], and can induce [[conditioned taste aversion]], where the brain associates the taste of a certain food with being toxic (usually after an individual consumes a food that had made them sick).
In obese individuals, GLP-1 secretion is decreased. When they lose weight, GLP-1 secretion returns to normal so GLP-1 might contribute to the pathogenesis of obesity. Accordingly, GLP-1 agonists have been targeted as a potential therapy for obesity; GLP-1 itself is not suitable as a clinical treatment for obesity as it has a very short half-life (about two minutes).


In obese individuals, GLP-1 secretion is decreased. When weight is lost in obese individuals GLP-1 secretion returns to normal (so GLP-1 could contribute to the pathogenesis of obesity). GLP-1 agonists have been targeted as a potential therapy for obesity. GLP-1 itself is not suitable as a clinical treatment for obesity as it has a very short half-life (about two minutes).
'''Peptide YY''' (PYY) is a 36-amino acid peptide secreted from endocrine cells (L-cells) in the [[ileum]] and [[colon]] <ref name=neary2009 />. PYY3-36 is dominant in the two endogenous forms in circulation with both affecting different Y-receptors found throughout the central nervous system, including the vagal nerve and within different brain regions. <ref name=karra2009>Karra E ''et al.'' (2009) The role of peptide YY in appetite regulation and obesity ''J Physiol'' 587:19-25 </ref> During fasting, plasma concentrations of PYY are low, but they increase after food intake, particularly high protein meals <ref name=batterham2006 /><ref name=tome2009>Tome ''et al.'' (2009) Protein, amino acids, vagus nerve signalling and the brain ''Am J Clin Nutr'' 90:838-43</ref>,(within 15 min) until a peak at 1-2 hours after the meal. <ref name=neary2009 /> PYY acts as an "ileal brake" to delay gastric emptying. PYY is mostly associated with [[satiety]] regulation, where high levels correlate with high satiety; <ref name=karra2009 /> it suppresses appetite in both rodents and humans <ref name=neary2009>Neary MT, Batterham RL (2009) Peptide YY: food for thought ''Physiol Behav'' 97:616-9</ref>. A correlation has been shown between high levels of PYY and low markers of adiposity and obese mice have low PYY levels in the circulation. <ref name=batterham2006>Batterham RL ''et al.'' (2006 ) Critical role for peptide YY in protein-mediated satiation and body-weight regulation. ''Cell Metab'' 4:223–33</ref>, and it is thought to be  involved in food intake reduction after [[bariatric surgery]]. <ref name=strader2005>Strader AD ''et al.''(2005) Weight loss through ileal transposition is accompanied by increased ileal hormone secretion and synthesis in rats ''Am  J Physiol'' 288:E447-53</ref> Gio ''et al.'' (2006) also found a negative correlation between PYY circulation levels and waist circumference, suggesting it as a contributing factor to energy expenditure and lipid metabolism. <ref name=Gio2006>Gio Y ''et al.''(2006) Physiological evidence for the involvement of peptide YY in the regulation of energy homeostasis in humans ''Obesity'' 14:1562-70</ref> PYY3-36 expression is  affected by other areas of the brain, including those connected with reward and pleasure - so PYY might be the ‘switch’ between homeostatic and [[Food reward|pleasure controlled ('hedonic') eating]]. <ref name=batterham2007>Batterham RL ''et al.'' (2007) PYY modulation of cortical and hypothalamic brain areas predicts feeding behaviour in humans ''Nature'' 450:106-9</ref>


'''Peptide YY''' (PYY) is a 36- amino acid peptide that is secreted from endocrine cells (L-cells) in the [[ileum]] and [[colon]], <ref name=neary2009 />. PYY3-36 is dominant in the two endogenous forms in circulation with both affecting different Y-receptors found through out the central nervous system, including the vagal nerve and within different brain regions. <ref name=karra2009>Karra E ''et al.'' (2009) The role of peptide YY in appetite regulation and obesity. ''J Physiol'' 587:19-25 </ref> During fasting, plasma concentrations of PYY are low, but they rapidly increase after food intake, particularly high protein meals <ref name=batterham2006 /><ref name=tome2009>Tome ''et al.'' (2009) Protein, amino acids, vagus nerve signalling and the brain ''Am J Clin Nutr'' 90:838-43</ref>,(within 15 min) until a peak at 1-2 hours after the meal. <ref name=neary2009 /> PYY acts as an "ileal brake" to delay gastric emptying. PYY is mostly associated with satiety regulation where high levels correlate with high satiety. <ref name=karra2009 /> It has been shown to reduce feeding in rodents and humans <ref name=neary2009>Neary MT, Batterham RL (2009) Peptide YY: food for thought ''Physiol Behav'' 97:616-9</ref>, a correlation has been proven between high levels of PYY and low markers of adiposity and Obese mice have been found to have low PYY circulation levels. <ref name=batterham2006>Batterham RL ''et al.'' (2006 ) Critical role for peptide YY in protein-mediated satiation and body-weight regulation. ''Cell Metab'' 4:223–33</ref> It is thought to be a factor involved in food intake reduction after [[bariatric surgery]]. <ref name=strader2005>Strader AD ''et al.''(2005) Weight loss through ileal transposition is accompanied by increased ileal hormone secretion and synthesis in rats. ''Am  J Physiol'' 288:E447-53
'''Insulin''' is a hormone secreted into the blood by [[pancreatic β-cell]]s in response to [[glucose]] intake. <ref name=woods1996>Woods SC ''et al.'' (1996) The evaluation of insulin as a metabolic signal influencing behaviour via the brain ''Neurosci Biobehav Rev'' 20:139-44</ref> Increased glucose causes secretion of other gut hormones, [[glucose-dependent insulinotrophic peptide]] (GIP) and GLP-1, which directly cause the secretion of insulin from the pancreas. <ref name=wook2008>Wook K, Egan JM (2008) The role of incretins in glucose and hemeostasis and diabetes treatment ''Pharmacol Rev'' 60:470-512</ref> With receptors in the hypothalamus (including the [[arcuate nucleus]]), [[cerebellum]], cortex and [[hippocampus]], insulin signalling in the brain causes a reduction in food intake and body weight <ref name=broberger2005>Broberger C (2005) Brain regulation of food intake and appetite: molecules and networks. ''J Int Med'' 258:301-27</ref> in the arcuate nucleus, it causes increased synthesis of POMC and inhibits production of the orexigenic neuropeptides [[neuropeptide Y]] (NPY) and [[agouti related peptide]] (AgRP), therefore suppressing appetite. <ref name=heijboer2006>Heijboer AC (2006) Insulin sensitivity: modulation by the gut-brain axis. </ref> Insulin also enhances the efficacy of systemic CCK in the brain to reduce overall food intake. <ref name=badman2005>Badman MK, Flier JS (2005) The gut and energy balance: visceral allies in the obesity wars ''Science'' 307:1909-14</ref> Reduced levels in insulin results in increased eating and weight gain.<ref name=woods2006>Woods SC ''et al.'' (2006) The brain-gut-islet connection ''Diabetes'' 55:114-21 </ref>
</ref> Gio ''et al.'' (2006) also found a negative correlation between PYY circulation levels and waist circumference, suggesting it as a contributing factor to energy expenditure and lipid metabolism. <ref name=Gio2006>Gio Y ''et al.''(2006) Physiological evidence for the involvement of peptide YY in the regulation of energy homeostasis in humans. ''Obesity'' 14:1562-70</ref>
PYY3-36 expression is  affected by other areas of the brain, namely those connected with reward and pleasure - PYY may be the ‘switch’ between homeostatic and pleasure controlled eating. <ref name=batterham2007>Batterham RL ''et al.'' (2007) PYY modulation of cortical and hypothalamic brain areas predicts feeding behaviour in humans ''Nature'' 450:106-9</ref>


'''Insulin''' is a hormone secreted into the blood by [[pancreatic β-cell]]s in response to [[glucose]] intake. <ref name=woods1996>Woods SC ''et al.'' (1996) The evaluation of insulin as a metabolic signal influencing behaviour via the brain ''Neurosci Biobehav Rev'' 20:139-44
Insulin has a long-term role in the control of energy homeostasis <ref name=badman2005 /> and metabolism. Over 24 hours, it is secreted at a mean level that is proportional to the size of fat stores in the body ([[adipocyte|adiposity]]). Insulin signalling in the arcuate nucleus and [[ventromedial nucleus]] directly affects the liver via the vagus nerve, inhibiting glucose production <ref name=heijboer2006 /> and facilitating glucose uptake by the muscles, liver and other tissues. It also promotes fat storage as [[triglyceride]]s and prevents fat breakdown thus inhibiting fat oxidation and further promoting glucose uptake in cells.  
</ref> Increased glucose causes secretion of other gut hormones, glucose-dependent insulinotrophic peptide (GIP) and glucagon-like peptide-1, which directly cause the secretion of insulin from the pancreas. <ref name=wook2008>Wook K, Egan JM (2008) The role of incretins in glucose and hemeostasis and diabetes treatment ''Pharmacol Rev'' 60:470-512
</ref> With receptors in the hypothalamus (including the [[arcuate nucleus]]), [[cerebellum]], cortex and [[hippocampus]], insulin signalling in the brain causes a reduction in food intake and body weight <ref name=broberger2005>Broberger C (2005) Brain regulation of food intake and appetite: molecules and networks. ''J Int Med'' 258:301-27</ref> in the arcuate nucleus, it causes increased synthesis of POMC and inhibits NPY and AgRP production, therefore increasing appetite inhibition.<ref name=heijboer2006>Heijboer AC (2006) Insulin sensitivity: modulation by the gut-brain axis. Department Endocrinology, Faculty of Medicine, Leiden University Medical Center, Leiden University.</ref> Insulin also enhances the efficacy of systemic CCK in the brain to reduce overall food intake. <ref name=badman2005>Badman MK, Flier JS (2005) The gut and energy balance: visceral allies in the obesity wars ''Science'' 307:1909-14
</ref> Reduced levels in insulin results in increased eating and weight gain.<ref name=woods2006>Woods SC ''et al.'' (2006) The Brain-Gut-Islet Connection ''Diabetes'' 55:114-21 </ref>


Insulin has an important long-term role in energy homeostasis <ref name=badman2005 /> and metabolism control. Its secretion levels correlate to the degree of adiposity within the subject<ref name=woods1996 /> and it acts as a continuous message to the brain concerning the amount of fat within the body <ref name=woods2006 /> and contributes to the long term regulation of body energy homeostasis. <ref name=woods2006 /> <ref name=woods1996 />
{{Image|diagram 4.jpg|right|500px|''Brain pathways'' Hormones entering the brain act on both POMC and neuropeptide Y neurons, resulting in altered food intake. Activation of neurons in the [[nucleus tractus solitarii]] affects other appetite and energy affecting systems}}
Insulin signalling in the ARC directly affects the liver via the vagus nerve, inhibiting endogenous glucose production <ref name=heijboer2006 /> and facilitating glucose uptake by the muscles, liver and other tissues. <ref name=woods2006 /> It also promotes fat storage as [[triglyceride]]s and prevents fat breakdown thus inhibiting fat oxidation and further promoting glucose uptake in cells.
'''Obestatin''', identified in 2005, is a sibling of ghrelin from preproghrelin.<ref name=guo2008>Guo Z ''et al.'' (2008) Different responses of circulating ghrelin, obestatin levels to fasting, re-feeding and different food compositions, and their local expressions in rats ''Peptides'' 29:1247-54</ref>  Obestatin is found in the stomach, with most obestatin-producing cells found in the basal part of the mucosa.<ref name=ren2009>Ren A ''et al.''(2009) Obestatin, obesity and diabetes. ''Peptides'' 30:439-44</ref> Obestatin inhibits food intake and therefore opposes the effects of ghrelin.  Obestatin reduces body weight gain, gastric empting, and also suppresses intestinal motility.<ref name=ren2009/>  Some studies suggest that obestatin also has further physiological functions including regulation of energy homeostasis, cell proliferation, hormone secretion and inhibition of thirst.<ref name=sheng2008>Sheng-Qui T ''et al.'' (2008) Obestatin: Its physicochemical characteristics and physiological functions. ''Peptides'' 29:639-65</ref>  Obestatin’s role in gut-brain signalling and the mechanisms that result in inhibition of appetite are still unclear.
 
{{Image|diagram 4.jpg|right|500px|''Brain pathways'' Hormones entering the brain act on POMC and NPY neurons, resulting in altered food intake. Activation of neurons in the [[nucleus tractus solitarii]] produces effects on other appetite and energy affecting systems}}
'''Obestatin''' is a sibling of ghrelin from preproghrelin identified in 2005.<ref name=guo2008>Guo Z ''et al.'' (2008) Different responses of circulating ghrelin, obestatin levels to fasting, re-feeding and different food compositions, and their local expressions in rats. Peptides 29:1247-1254</ref>  Obestatin is found in the stomach, with most obestatin-producing cells found in the basal part of the mucosa.<ref name=ren2009>Ren A ''et al.''(2009) Obestatin, obesity and diabetes. ''Peptides'' 30:439-44</ref> Obestatin inhibits food intake and therefore opposes the effects of ghrelin.  Obestatin reduces body weight gain, gastric empting, and also suppresses intestinal motility.<ref name=ren2009 />  Some studies suggest that obestatin also has further physiological functions including regulation of energy homeostasis, cell proliferation, hormone secretion and inhibition of thirst.<ref name=sheng2008>Sheng-Qui T ''et al.'' (2008) Obestatin: Its physicochemical characteristics and physiological functions. ''Peptides'' 29:639-65</ref>  Obestatin’s role in gut-brain signalling and the mechanisms that result in its inhibition of appetite are still unclear.


==Orexigenic Signals==
==Orexigenic Signals==
'''[[Ghrelin]]''' is a 28-amino acid brain-gut peptide -cleaved from preproghrelin.<ref name=cummings2006>Cummings D. (2006) Ghrelin and the short- and long- term regulation of appetite and body weight. ''Physiol Behav'' 89:71-84</ref> It is synthesised and secreted primarily by endocrine cells in the stomach, with appetite-inducing activities.<ref name=korbonits2004>Korbonits M. et al (2004) Ghrelin—a hormone with multiple functions. ''Front Neuroendocrinol'' 25:27-68</ref><ref name=geary2004>Geary N (2004) Endocrine controls of eating: CCK, leptin and ghrelin. ''Physiol Behav'' 81:719-33</ref>  Initially Ghrelin was identified as an endogenous ligand for the growth hormone secretagogue receptor (GHS-R).<ref name=cummings2006 />  Endogenous levels of ghrelin change according to nutritional status; with levels increasing during fasting and immediately decreasing after food intake, providing evidence that it may be involved in food initiation. Administration of ghrelin (centrally and peripherally) results in appetite stimulation and increased food intake. Circulating concentrations of ghrelin are lower in obese people and are negatively correlated with [[body mass index]].
'''[[Ghrelin]]''' is a 28-amino acid brain-gut peptide -cleaved from preproghrelin.<ref name=cummings2006>Cummings D. (2006) Ghrelin and the short- and long- term regulation of appetite and body weight ''Physiol Behav'' 89:71-84</ref> It is synthesised and secreted primarily by endocrine cells in the stomach, with appetite-inducing activities.<ref name=korbonits2004>Korbonits M ''et al.'' (2004) Ghrelin—a hormone with multiple functions. ''Front Neuroendocrinol'' 25:27-68</ref><ref name=geary2004>Geary N (2004) Endocrine controls of eating: CCK, leptin and ghrelin ''Physiol Behav'' 81:719-33</ref>  Initially Ghrelin was identified as an endogenous ligand for the growth hormone secretagogue receptor (GHS-R).<ref name=cummings2006 />  Endogenous levels of ghrelin change according to nutritional status; with levels increasing during fasting and immediately decreasing after food intake, providing evidence that it may be involved in food initiation. Administration of ghrelin (centrally and peripherally) results in appetite stimulation and increased food intake. Circulating concentrations of ghrelin are lower in obese people and are negatively correlated with [[body mass index]].
    
    
Ghrelin can cross the [[blood-brain barrier]], and its effects on eating behaviour are thought to be mediated mainly via the arcuate nucleus, where it activates neurons that express the appetite stimulating peptides [[neuropeptide Y]] (NPY) and [[agouti-related peptide]] (AgRP).<ref name=naslund2007>Näslund E ''et al.'' (2007) Appetite signaling: from gut peptides and enteric nerves to brain. ''Physiol Behav'' 92:256-62</ref>
Ghrelin's effects on eating behaviour are thought to be mediated mainly via the arcuate nucleus, where it activates neurons that express the appetite stimulating neuropeptides NPY and AgRP.<ref name=naslund2007>Näslund E ''et al.'' (2007) Appetite signaling: from gut peptides and enteric nerves to brain ''Physiol Behav'' 92:256-62</ref>


However, the receptors through which ghrelin acts are expressed in some other parts of the brain and in peripheral tissues, and ghrelin also influences many other biological actions including effects such as gastrointestinal (GI) motility-stimulating gastric emptying, cardiovascular function, immunity, inflammation, pancreatic function, hormone secretion and memory among others.<ref name=cummings2006 /> <ref name=cummings2006 /> It is still unclear by what mechanism and to what extent ghrelin plays a role in food initiation, and some of ghrelin's orexigenic activities may involve the gastric afferent vagal nerve.
However, the receptors through which ghrelin acts are expressed in some other parts of the brain and in peripheral tissues, and ghrelin also influences many other biological actions including effects such as gastrointestinal motility-stimulating gastric emptying, cardiovascular function, immunity, inflammation, pancreatic function, hormone secretion and memory among others.<ref name=cummings2006 /><ref name=cummings2006/> It is still unclear how and to what extent ghrelin plays a role in food initiation.


==Role of the Vagus Nerve==
==Role of the Vagus Nerve==


Signaling from the gut can be both neural and hormonal.  Neural communication from the gut begins with the stomach, where vagal afferent fibres are widely distributed to detect distension of the stomach walls <ref name=Berthoud2008>Berthoud HR (2008) Vagal and hormonal gut–brain communication: from satiation to satisfaction.''Neurogastroenterol Motil'' 20:64–72 </ref>. The hormones released by the stomach and intestines  reach the brain via the circulation or receptor activation on vagal afferent neurons <ref name=Berthoud2008 />.  There have been many identified receptors present on the afferent neurons which can allow for this, including the CCK1 receptor for cholecystokinin, the PYY receptor Y2, and GHS-1 which binds ghrelin.  The vagal afferent neurons alter the expression of receptors on the membrane in relation to CCK concentrations; administration of CCK to fasted rats increased the expression of the Y2 receptor, sensitizing the vagal afferent neurons to appetite signaling and satiety signaling.<ref name=Dockray2009>Dockray GJ (2009)The versatility of the vagus ''Physiol Behav'' 97:531-6 </ref>
Signaling from the gut can be both neural and hormonal.  Neural communication from the gut begins with the stomach, where vagal afferent fibres are widely distributed to detect distension of the stomach walls <ref name=Berthoud2008>Berthoud HR (2008) Vagal and hormonal gut–brain communication: from satiation to satisfaction ''Neurogastroenterol Motil'' 20:64–72 </ref>. The hormones released by the stomach and intestines  reach the brain directly via the circulation, where they are sensed at sites where the blood-brain barrier is permeable, and by activating vagal afferent neurons <ref name=Berthoud2008/>.  Thus vagal afferents possess the CCK1 receptor for cholecystokinin, and receptors for glucagon-like peptide 1, and the PYY receptor (Y2), and the GHS-1 receptor (which binds ghrelin) are expressed in the arcuate nucleus of the hypothalamus as well as on vagal afferent nerve endings.  The vagal afferent neurones alter the expression of receptors on the membrane in relation to CCK concentrations; administration of CCK to fasted rats increased the expression of Y2 receptors, sensitizing the vagal afferent neurons to appetite signaling and satiety signaling.<ref name=Dockray2009>Dockray GJ (2009)The versatility of the vagus ''Physiol Behav'' 97:531-6 </ref>


==Neuropeptide Y (NPY) and Agouti-Related Peptide==
==Neuropeptide Y (NPY) and Agouti Related Peptide (AgRP)==
[[Neuropeptide Y]] (NPY) and [[agouti-related peptide]] (AgRP) are both potent stimulators of appetite.  These neuropeptides are co-expressed in a subpopulation of neurons in the arcuate nucleus of the hypothalamus <ref name=Smith2008>Smith PM, Ferguson AV (2008)Neurophysiology of hunger and satiety ''Dev Disabilities Res Rev'' 14:96–104 </ref>.  AgRP is an endogenous inverse agonist of [[melanocortin receptor]]s <ref name=Kas2005>Kas MJH ''et al.''(2005)Differential regulation of agouti-related protein and neuropeptide Y in hypothalamic neurons following a stressful event. ''J Mol Endocrinol'' 35:159-64 </ref>. One key mechanism by which NYP/AgRP neurons increase appetite is by antagonising the actions of [[α-MSH]] (produced by POMC cells of the arcuate nucleus) by binding to MC4R receptors <ref name=Luquet2005>Luquet S ''et al.''(2005)NPY/AgRP neurons are essential for feeding in adult mice but can be ablated in neonates. ''Science'' 310683-5 </ref>, as α-MSH is the most potent appetite inhibitor to be found. ''Luquet et al.'' selectively ablated the NPY/AGRP neurons  using transgenic mice in which the human diphtheria toxin receptor was targetted to be expressed by AgRP-expressing neurons. If this ablation was done in neonates, the mice could compensate for the loss of neurons, and by the time they reached adulthood they had normal body weight and appetite. However when neurons were ablated in adult mice, they lost all appetite.
NPY and AgRP are co-expressed in a subpopulation of neurones in the arcuate nucleus of the hypothalamus, and are both potent stimulators of appetite. <ref name=Smith2008>Smith PM, Ferguson AV (2008) Neurophysiology of hunger and satiety ''Dev Disabilities Res Rev'' 14:96–104</ref>.  AgRP is an inverse agonist of [[melanocortin receptor]]s <ref name=Kas2005>Kas MJH ''et al.''(2005) Differential regulation of agouti-related protein and neuropeptide Y in hypothalamic neurons following a stressful event ''J Mol Endocrinol'' 35:159-64 </ref>. One key mechanism by which NYP/AgRP neurones increase appetite is by antagonising the actions of [[α-MSH]] (produced by POMC cells of the arcuate nucleus) by binding to MC4 receptors <ref name=Luquet2005>Luquet S ''et al.''(2005)NPY/AgRP neurones are essential for feeding in adult mice but can be ablated in neonates ''Science'' 310683-5</ref>, as α-MSH is a very potent appetite inhibitor. Luquet ''et al.'' selectively ablated the NPY/AgRP neurones using transgenic mice in which the human diphtheria toxin receptor was targetted to be expressed by AgRP-expressing neurones. If this ablation was done in neonates, the mice could compensate for the loss of neurones, and by the time they reached adulthood they had normal body weight and appetite. However when the neurones were ablated in adult mice, the mice lost all appetite.


==References==
==References==
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Gut-brain signalling describes the interaction between the gastrointestinal tract and the brain, and how secretion of varying hormones from different areas of the body causes appetite-enhancing and satiety signals to be sent to the brain. The hormones that have been most intensely studied are: ghrelin, obestatin, cholecystokinin (CCK), glucagon-like peptide-1 (GLP-1), peptide YY (PYY) and insulin which all have roles in appetite regulation. The vagus nerve is also a key mediator of regulation, and all of these inputs are processed by areas in the brain such as the hypothalamus and the nucleus tractus solitarii (NTS).

Anorexic Signals

Gut-Brain signaling Pathways Proteins and hormones activate brain pathways in different ways, either by eventual vagal activation or through peripheral circulation. The nucleus tractus solitarii and the arcuate nucleus are then activated.

Cholecystokinin (CCK) is a hormone synthesised by L-cells in the mucosal epithelium of the duodenum, and secreted in response to the presence of partly digested lipids and proteins. CCK inhibits gastric emptying and stimulates the release of digestive enzymes from the pancreas and bile from the gall bladder by acting at CCK-A receptors on afferent vagal nerve endings in the gastrointestinal tract. Because gastric emptying is inhibited, the partly digested lipids and proteins are exposed to the digestive enzymes and bile so are further broken down. As the lipids and proteins are broken down, CCK secretion declines.

CCK is a ‘gatekeeper’ for the response of other gut-brain signalling hormones on the afferent vagal neurons. At low levels (after fasting), CCK stimulates the expression of receptors associated with the stimulation of food intake, including receptors for melanin concentrating hormone (MCH)-1 and cannabinoid CB1 receptors. At high levels (after food consumption), MCH-1 and CB1 receptors are down-regulated. Therefore CCK, at a high or low concentration, can affect how afferent vagal neurons respond to other neurohormones.

Glucagon-like peptide-1 (GLP-1) is a hormone secreted from L-cells in the mucosal epithelium of the duodenum and small intestine. It is derived from the pro-glucagon gene, and is secreted into the circulation in response to the presence of nutrients. It acts at the pancreas, where it stimulates insulin secretion and suppresses glucagon secretion; it also increases insulin sensitivity. GLP-1 also activates anorexigenic neurons in the arcuate nucleus via the caudal brainstem, thereby suppressing appetite. It also decreases gastric emptying, so adds to the feeling of being ‘full’. At higher concentrations, GLP-1 causes nausea, and can induce conditioned taste aversion, where the brain associates the taste of a certain food with being toxic (usually after an individual consumes a food that had made them sick).

In obese individuals, GLP-1 secretion is decreased. When they lose weight, GLP-1 secretion returns to normal so GLP-1 might contribute to the pathogenesis of obesity. Accordingly, GLP-1 agonists have been targeted as a potential therapy for obesity; GLP-1 itself is not suitable as a clinical treatment for obesity as it has a very short half-life (about two minutes).

Peptide YY (PYY) is a 36-amino acid peptide secreted from endocrine cells (L-cells) in the ileum and colon [1]. PYY3-36 is dominant in the two endogenous forms in circulation with both affecting different Y-receptors found throughout the central nervous system, including the vagal nerve and within different brain regions. [2] During fasting, plasma concentrations of PYY are low, but they increase after food intake, particularly high protein meals [3][4],(within 15 min) until a peak at 1-2 hours after the meal. [1] PYY acts as an "ileal brake" to delay gastric emptying. PYY is mostly associated with satiety regulation, where high levels correlate with high satiety; [2] it suppresses appetite in both rodents and humans [1]. A correlation has been shown between high levels of PYY and low markers of adiposity and obese mice have low PYY levels in the circulation. [3], and it is thought to be involved in food intake reduction after bariatric surgery. [5] Gio et al. (2006) also found a negative correlation between PYY circulation levels and waist circumference, suggesting it as a contributing factor to energy expenditure and lipid metabolism. [6] PYY3-36 expression is affected by other areas of the brain, including those connected with reward and pleasure - so PYY might be the ‘switch’ between homeostatic and pleasure controlled ('hedonic') eating. [7]

Insulin is a hormone secreted into the blood by pancreatic β-cells in response to glucose intake. [8] Increased glucose causes secretion of other gut hormones, glucose-dependent insulinotrophic peptide (GIP) and GLP-1, which directly cause the secretion of insulin from the pancreas. [9] With receptors in the hypothalamus (including the arcuate nucleus), cerebellum, cortex and hippocampus, insulin signalling in the brain causes a reduction in food intake and body weight [10] in the arcuate nucleus, it causes increased synthesis of POMC and inhibits production of the orexigenic neuropeptides neuropeptide Y (NPY) and agouti related peptide (AgRP), therefore suppressing appetite. [11] Insulin also enhances the efficacy of systemic CCK in the brain to reduce overall food intake. [12] Reduced levels in insulin results in increased eating and weight gain.[13]

Insulin has a long-term role in the control of energy homeostasis [12] and metabolism. Over 24 hours, it is secreted at a mean level that is proportional to the size of fat stores in the body (adiposity). Insulin signalling in the arcuate nucleus and ventromedial nucleus directly affects the liver via the vagus nerve, inhibiting glucose production [11] and facilitating glucose uptake by the muscles, liver and other tissues. It also promotes fat storage as triglycerides and prevents fat breakdown thus inhibiting fat oxidation and further promoting glucose uptake in cells.

Brain pathways Hormones entering the brain act on both POMC and neuropeptide Y neurons, resulting in altered food intake. Activation of neurons in the nucleus tractus solitarii affects other appetite and energy affecting systems

Obestatin, identified in 2005, is a sibling of ghrelin from preproghrelin.[14] Obestatin is found in the stomach, with most obestatin-producing cells found in the basal part of the mucosa.[15] Obestatin inhibits food intake and therefore opposes the effects of ghrelin. Obestatin reduces body weight gain, gastric empting, and also suppresses intestinal motility.[15] Some studies suggest that obestatin also has further physiological functions including regulation of energy homeostasis, cell proliferation, hormone secretion and inhibition of thirst.[16] Obestatin’s role in gut-brain signalling and the mechanisms that result in inhibition of appetite are still unclear.

Orexigenic Signals

Ghrelin is a 28-amino acid brain-gut peptide -cleaved from preproghrelin.[17] It is synthesised and secreted primarily by endocrine cells in the stomach, with appetite-inducing activities.[18][19] Initially Ghrelin was identified as an endogenous ligand for the growth hormone secretagogue receptor (GHS-R).[17] Endogenous levels of ghrelin change according to nutritional status; with levels increasing during fasting and immediately decreasing after food intake, providing evidence that it may be involved in food initiation. Administration of ghrelin (centrally and peripherally) results in appetite stimulation and increased food intake. Circulating concentrations of ghrelin are lower in obese people and are negatively correlated with body mass index.

Ghrelin's effects on eating behaviour are thought to be mediated mainly via the arcuate nucleus, where it activates neurons that express the appetite stimulating neuropeptides NPY and AgRP.[20]

However, the receptors through which ghrelin acts are expressed in some other parts of the brain and in peripheral tissues, and ghrelin also influences many other biological actions including effects such as gastrointestinal motility-stimulating gastric emptying, cardiovascular function, immunity, inflammation, pancreatic function, hormone secretion and memory among others.[17][17] It is still unclear how and to what extent ghrelin plays a role in food initiation.

Role of the Vagus Nerve

Signaling from the gut can be both neural and hormonal. Neural communication from the gut begins with the stomach, where vagal afferent fibres are widely distributed to detect distension of the stomach walls [21]. The hormones released by the stomach and intestines reach the brain directly via the circulation, where they are sensed at sites where the blood-brain barrier is permeable, and by activating vagal afferent neurons [21]. Thus vagal afferents possess the CCK1 receptor for cholecystokinin, and receptors for glucagon-like peptide 1, and the PYY receptor (Y2), and the GHS-1 receptor (which binds ghrelin) are expressed in the arcuate nucleus of the hypothalamus as well as on vagal afferent nerve endings. The vagal afferent neurones alter the expression of receptors on the membrane in relation to CCK concentrations; administration of CCK to fasted rats increased the expression of Y2 receptors, sensitizing the vagal afferent neurons to appetite signaling and satiety signaling.[22]

Neuropeptide Y (NPY) and Agouti Related Peptide (AgRP)

NPY and AgRP are co-expressed in a subpopulation of neurones in the arcuate nucleus of the hypothalamus, and are both potent stimulators of appetite. [23]. AgRP is an inverse agonist of melanocortin receptors [24]. One key mechanism by which NYP/AgRP neurones increase appetite is by antagonising the actions of α-MSH (produced by POMC cells of the arcuate nucleus) by binding to MC4 receptors [25], as α-MSH is a very potent appetite inhibitor. Luquet et al. selectively ablated the NPY/AgRP neurones using transgenic mice in which the human diphtheria toxin receptor was targetted to be expressed by AgRP-expressing neurones. If this ablation was done in neonates, the mice could compensate for the loss of neurones, and by the time they reached adulthood they had normal body weight and appetite. However when the neurones were ablated in adult mice, the mice lost all appetite.

References

  1. ↑ 1.0 1.1 1.2 Neary MT, Batterham RL (2009) Peptide YY: food for thought Physiol Behav 97:616-9
  2. ↑ 2.0 2.1 Karra E et al. (2009) The role of peptide YY in appetite regulation and obesity J Physiol 587:19-25
  3. ↑ 3.0 3.1 Batterham RL et al. (2006 ) Critical role for peptide YY in protein-mediated satiation and body-weight regulation. Cell Metab 4:223–33
  4. ↑ Tome et al. (2009) Protein, amino acids, vagus nerve signalling and the brain Am J Clin Nutr 90:838-43
  5. ↑ Strader AD et al.(2005) Weight loss through ileal transposition is accompanied by increased ileal hormone secretion and synthesis in rats Am J Physiol 288:E447-53
  6. ↑ Gio Y et al.(2006) Physiological evidence for the involvement of peptide YY in the regulation of energy homeostasis in humans Obesity 14:1562-70
  7. ↑ Batterham RL et al. (2007) PYY modulation of cortical and hypothalamic brain areas predicts feeding behaviour in humans Nature 450:106-9
  8. ↑ Woods SC et al. (1996) The evaluation of insulin as a metabolic signal influencing behaviour via the brain Neurosci Biobehav Rev 20:139-44
  9. ↑ Wook K, Egan JM (2008) The role of incretins in glucose and hemeostasis and diabetes treatment Pharmacol Rev 60:470-512
  10. ↑ Broberger C (2005) Brain regulation of food intake and appetite: molecules and networks. J Int Med 258:301-27
  11. ↑ 11.0 11.1 Heijboer AC (2006) Insulin sensitivity: modulation by the gut-brain axis.
  12. ↑ 12.0 12.1 Badman MK, Flier JS (2005) The gut and energy balance: visceral allies in the obesity wars Science 307:1909-14
  13. ↑ Woods SC et al. (2006) The brain-gut-islet connection Diabetes 55:114-21
  14. ↑ Guo Z et al. (2008) Different responses of circulating ghrelin, obestatin levels to fasting, re-feeding and different food compositions, and their local expressions in rats Peptides 29:1247-54
  15. ↑ 15.0 15.1 Ren A et al.(2009) Obestatin, obesity and diabetes. Peptides 30:439-44
  16. ↑ Sheng-Qui T et al. (2008) Obestatin: Its physicochemical characteristics and physiological functions. Peptides 29:639-65
  17. ↑ 17.0 17.1 17.2 17.3 Cummings D. (2006) Ghrelin and the short- and long- term regulation of appetite and body weight Physiol Behav 89:71-84
  18. ↑ Korbonits M et al. (2004) Ghrelin—a hormone with multiple functions. Front Neuroendocrinol 25:27-68
  19. ↑ Geary N (2004) Endocrine controls of eating: CCK, leptin and ghrelin Physiol Behav 81:719-33
  20. ↑ Näslund E et al. (2007) Appetite signaling: from gut peptides and enteric nerves to brain Physiol Behav 92:256-62
  21. ↑ 21.0 21.1 Berthoud HR (2008) Vagal and hormonal gut–brain communication: from satiation to satisfaction Neurogastroenterol Motil 20:64–72
  22. ↑ Dockray GJ (2009)The versatility of the vagus Physiol Behav 97:531-6
  23. ↑ Smith PM, Ferguson AV (2008) Neurophysiology of hunger and satiety Dev Disabilities Res Rev 14:96–104
  24. ↑ Kas MJH et al.(2005) Differential regulation of agouti-related protein and neuropeptide Y in hypothalamic neurons following a stressful event J Mol Endocrinol 35:159-64
  25. ↑ Luquet S et al.(2005)NPY/AgRP neurones are essential for feeding in adult mice but can be ablated in neonates Science 310683-5
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