Electrophoresis: Difference between revisions
Jump to navigation
Jump to search
imported>Sarah Richardson No edit summary |
imported>Sarah Richardson No edit summary |
||
| Line 4: | Line 4: | ||
'''Electrophoresis''' is a separation technique frequently used in the analysis of proteins and nucleic acids. The process known as electrophoresis, involves the migration of particles or molecules (in particular proteins, DNA, and RNA) through an electric field that separates them exclusively on the basis of their size or molecular weight. The direction the molecule moves depends on its charge while the rate of migration is affected by the size, shape, density of the gel and the strength of the applied current (5c). | '''Electrophoresis''' is a separation technique frequently used in the analysis of proteins and nucleic acids. The process known as electrophoresis, involves the migration of particles or molecules (in particular proteins, DNA, and RNA) through an electric field that separates them exclusively on the basis of their size or molecular weight. The direction the molecule moves depends on its charge while the rate of migration is affected by the size, shape, density of the gel and the strength of the applied current (5c). | ||
Electrophoresis is a very simple process and relatively quick with a high resolution. In addition electrophoresis is an extremely useful method to estimate the purity of a sample. The technique is also very sensitive to slight variations in molecular weight, size, and even shape of nucleic acids and proteins <ref name=Harrison>Harrison, R. G., Todd, P., Rudge S. R., Petrides, D. P. (2003). Bioseparations Science and Engineering. New York, NY: Oxford University Press.</ref>. Electrophoresis can also be useful when it doesn’t affect the molecule’s structure or denature the protein <ref name = Harrison /ref>. | Electrophoresis is a very simple process and relatively quick with a high resolution. In addition electrophoresis is an extremely useful method to estimate the purity of a sample. The technique is also very sensitive to slight variations in molecular weight, size, and even shape of nucleic acids and proteins <ref name=Harrison>Harrison, R. G., Todd, P., Rudge S. R., Petrides, D. P. (2003). Bioseparations Science and Engineering. New York, NY: Oxford University Press.</ref>. Electrophoresis can also be useful when it doesn’t affect the molecule’s structure or denature the protein <ref name = Harrison /ref>. | ||
==Apparatus== | |||
==The Process== | |||
As shown in the diagram, the nucleic acids or proteins are loaded into the wells or depressions at one end on the eletrophoretic medium (also known as a gel). The apparatus also has two electrodes on either side of the eletrophoretic medium. The anode is positively charged while the cathode is negatively charged. When a power source connects the two electrodes the charged particles begin to migrate towards the oppositely charged electrode due to the electric potential field within the media. <ref name=Harrison /ref> | |||
==History== | |||
This section should describe the invention and development of the process. If the section runs long, divide it into chronological subsections, for example: | |||
===Invention and early development=== | |||
This subsection should provide some historical context for the development of your process, describe its invention, and name some early developers and/or applications. | |||
===Recent developments=== | |||
This section should discuss new developments in the field. Don't hesitate to drop in brief mentions of processes or features you don't intend to discuss in depth. By so doing you are planting seeds of articles which will eventually be developed by others.<ref>"New Directions for Flocculation," American Flocculation Society. 2006. Retrieved July 21, 2009 from [http://www.amflocsoc.org/future_devs.html http://www.amflocsoc.org/future_devs.html]</ref> | |||
==Operation== | |||
Use lots of subsections here as you describe various aspects of the process . | |||
==Applications== | |||
This section should discuss how the process is used in practice. | |||
==Examples== | |||
If you have used a lot of equations in your article, this may be a good place to show an example of how they are used. See the article on the Antoine Equation for an example. | |||
==References== | |||
<references/> | |||
Revision as of 17:04, 29 November 2010
 For the course duration, the article is closed to outside editing. Of course you can always leave comments on the discussion page. The anticipated date of course completion is 21 December 2010. One month after that date at the latest, this notice shall be removed. Besides, many other Citizendium articles welcome your collaboration! Note to course participants: Looking forward to some insightful and useful articles from your collaborations. |
Electrophoresis is a separation technique frequently used in the analysis of proteins and nucleic acids. The process known as electrophoresis, involves the migration of particles or molecules (in particular proteins, DNA, and RNA) through an electric field that separates them exclusively on the basis of their size or molecular weight. The direction the molecule moves depends on its charge while the rate of migration is affected by the size, shape, density of the gel and the strength of the applied current (5c).
Electrophoresis is a very simple process and relatively quick with a high resolution. In addition electrophoresis is an extremely useful method to estimate the purity of a sample. The technique is also very sensitive to slight variations in molecular weight, size, and even shape of nucleic acids and proteins [1]. Electrophoresis can also be useful when it doesn’t affect the molecule’s structure or denature the protein Cite error: Invalid <ref> tag; invalid names, e.g. too many
Operation
Use lots of subsections here as you describe various aspects of the process .
Applications
This section should discuss how the process is used in practice.
Examples
If you have used a lot of equations in your article, this may be a good place to show an example of how they are used. See the article on the Antoine Equation for an example.
References
- ↑ Harrison, R. G., Todd, P., Rudge S. R., Petrides, D. P. (2003). Bioseparations Science and Engineering. New York, NY: Oxford University Press.