Flow cytometry: Difference between revisions
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'''Flow cytometry (FC)''' is a laser-based fluorescence detection method used to characterize cell antigens on large numbers of [[blood cells|hematopoetic]] or [[lymphoid cell]]s in suspension typically derived from peripheral blood, bone marrow aspirate or lymph node tissue. Flow cytometry instruments use a thin laminar flow of fluid to direct thousands of cells from a small diameter nozzle through a thin beam of laser light of a certain wavelength. The cells are categorized by size forward light scatter, cellular complexity or granularity side scatter and by antigen density fluorescence intensity for each 'CD' clusters of differentiation marker. The antigen distribution for thousands of cells is printed in graphical histograms which a pathologist interprets individually and collectively to determine the phenotype of dominant cell populations in [[leukemia]]s and [[lymphoma]]s of various types. | '''Flow cytometry (FC)''' is a laser-based fluorescence detection method used to characterize cell antigens on large numbers of [[blood cells|hematopoetic]] or [[lymphoid cell]]s in suspension typically derived from peripheral blood, bone marrow aspirate or lymph node tissue.<ref>{{citation | ||
| url = http://depts.washington.edu/labweb/Divisions/Hema/Flow.htm | |||
| title = Division of Laboratory Medicine, [[University of Washington]] | |||
| title = Flow Cytometry}}</ref> Flow cytometry instruments use a thin laminar flow of fluid to direct thousands of cells from a small diameter nozzle through a thin beam of laser light of a certain wavelength. The cells are categorized by size forward light scatter, cellular complexity or granularity side scatter and by antigen density fluorescence intensity for each 'CD' clusters of differentiation marker. The antigen distribution for thousands of cells is printed in graphical histograms which a pathologist interprets individually and collectively to determine the phenotype of dominant cell populations in [[leukemia]]s and [[lymphoma]]s<ref>{{citation | |||
| journal = Journal of Immunological Methods | |||
| volume=171 |issue=1| date = 2 May 1994| pages=131-137 | |||
| author = A.Bruce Lyons and Christopher R. Parisha | |||
| title = (Abstract) Determination of lymphocyte division by flow cytometry | |||
| doi = 10.1016/0022-1759(94)90236-4 | |||
| url = http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2Y-476MXPW-14K&_user=10&_coverDate=05%2F02%2F1994&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1418026211&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=868e2001b89b6b687eb413d177e6fb38 | |||
}}</ref> of various types. | |||
==References== | ==References== | ||
<references/> | <references/> | ||
Revision as of 15:54, 1 August 2010
Flow cytometry (FC) is a laser-based fluorescence detection method used to characterize cell antigens on large numbers of hematopoetic or lymphoid cells in suspension typically derived from peripheral blood, bone marrow aspirate or lymph node tissue.[1] Flow cytometry instruments use a thin laminar flow of fluid to direct thousands of cells from a small diameter nozzle through a thin beam of laser light of a certain wavelength. The cells are categorized by size forward light scatter, cellular complexity or granularity side scatter and by antigen density fluorescence intensity for each 'CD' clusters of differentiation marker. The antigen distribution for thousands of cells is printed in graphical histograms which a pathologist interprets individually and collectively to determine the phenotype of dominant cell populations in leukemias and lymphomas[2] of various types.
References
- ↑ Flow Cytometry
- ↑ A.Bruce Lyons and Christopher R. Parisha (2 May 1994), "(Abstract) Determination of lymphocyte division by flow cytometry", Journal of Immunological Methods 171 (1): 131-137, DOI:10.1016/0022-1759(94)90236-4