Caenorhabditis elegans

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Caenorhabditis elegans
Scientific classification
Kingdom: Animalia Phylum: Nematoda Class: Secernentea Order: Rhabditida Family: Rhabditidae Genus: Caenorhabdtis Species: C.elegans
Binomial name
Caenorhabdtis elegans Maupas 1900

Description and significance

Caenorhabditis elegan is a simple organism that is small free living nematode. It is found in various parts of the world. A microscope is required to view a C. elegans because its length ranges up to 1 mm. The growth of C. elegans is rapid; under a standard laboratory condition it takes the entire life cycle from egg to an adult producing more eggs in just 3.5 days at 20°C.¹ After the egg hatches, it undergoes four larval stages L1 to L4; the larval stages are punctuated by molts.² After the L4 stage the C. elegan will undergo a final molt to produce an adult.¹ It is an adult stage that its length is 1mm. At a time when there is a food shortage and a high population, the C. elegans take an alternative pathway during the larval stages. At the alternative stage a dauer is formed after the molt of L2 instead of a L3. The dauer is able to survive these conditions by not feeding and is resistant to desiccation.² The dauer stage is able to survive for several months without further developing; when there is change in the environment such as a availability of food the C. elegan will molt to the normal L4 stage. C. elegans lives for about 18- 23 days. There are two types of sexes which are hermaphrodites and males; its appearance is visually different at the adult stage. The male only produces sperm, in order to reproduce it must mate with a hermaphrodite. This cross fertilization produces both the males and hermaphrodites in a 1:1 proportion. The hermaphrodites are able to produce both sperm and oocytes and undergo self-fertilization. During the self-fertilization it produces hermaphrodites and males arise spontaneously at an extremely low frequency.

The C. elegans are part of the model system. The key attributes of C. elegans as an experimental system for biological systems are its simplicity, transparency, ease of cultivation in the laboratory, short life cycle, suitability of genetic analysis and small genome size.² Its biological mechanisms are similar to majority of the animal species that allows an ideal model system.

Genome structure

The genes control all the functions and proper developments of the C.elegans. It is one of the first multicellular organisms that have the entire genome sequenced. The C. elegan has a small genome, yet a very complex one. It has over 19,000 protein coding genes.¹ There are 8 x 107 base pairs of DNA in the genome.³ In the genome there are many mutable loci; that allow observing the genetic defects easier therefore making it an model organism. The C.elegans cells are diploid. Both the hermaphrodites and males have five autosomes; the only difference being that the hermaphrodites have one pair X chromsomes while the male has one X chromosome.

References

1. Hope I.A, ed. C. elegans: A Practical Approach. New York: Oxford University Press, 1999.

2. Wood William Barry; ed. The Nematode Caenorhabditis Elegans. New York: Cold Spring Harbor Laboratory, 1988.

3. Riddle Donald, Blumenthal Thomas, Meyer Barbara, Priess James eds. C. elegans II. New York: Cold Spring Harbor Laboratory, 1997.