Caenorhabditis elegans

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Caenorhabditis elegans
Scientific classification
Kingdom: Animalia Phylum: Nematoda Class: Secernentea Order: Rhabditida Family: Rhabditidae Genus: Caenorhabdtis Species: C.elegans
Binomial name
Caenorhabdtis elegans Maupas 1900

Description and significance

Caenorhabditis elegan is a simple organism that is small free living nematode. It is found in various parts of the world. A microscope is required to view a C. elegans because its length ranges up to 1 mm. The growth of C. elegans is rapid; under a standard laboratory condition it takes the entire life cycle from egg to an adult producing more eggs in just 3.5 days at 20°C.¹ After the egg hatches, it undergoes four larval stages L1 to L4; the larval stages are punctuated by molts.² After the L4 stage the C. elegan will undergo a final molt to produce an adult.¹ It is an adult stage that its length is 1mm. At a time when there is a food shortage and a high population, the C. elegans take an alternative pathway during the larval stages. At the alternative stage a dauer is formed after the molt of L2 instead of a L3. The dauer is able to survive these conditions by not feeding and is resistant to desiccation.² The dauer stage is able to survive for several months without further developing; when there is change in the environment such as a availability of food the C. elegan will molt to the normal L4 stage. C. elegans lives for about 18- 23 days. There are two types of sexes which are hermaphrodites and males; its appearance is visually different at the adult stage. The male only produces sperm, in order to reproduce it must mate with a hermaphrodite. This cross fertilization produces both the males and hermaphrodites in a 1:1 proportion. The hermaphrodites are able to produce both sperm and oocytes and undergo self-fertilization. During the self-fertilization it produces hermaphrodites and males arise spontaneously at an extremely low frequency.

The C. elegans are part of the model system. The key attributes of C. elegans as an experimental system for biological systems are its simplicity, transparency, ease of cultivation in the laboratory, short life cycle, suitability of genetic analysis and small genome size.² Its biological mechanisms are similar to majority of the animal species that allows an ideal model system.

Genome structure

The genes control all the functions and proper developments of the C.elegans. It is one of the first multicellular organisms that have the entire genome sequenced. The C. elegan has a small genome, yet a very complex one. It has over 19,000 protein coding genes.¹ There are 8 x 107 base pairs of DNA in the genome.³ In the genome there are many mutable loci; that allow observing the genetic defects easier therefore making it an model organism. The C.elegans cells are diploid. Both the hermaphrodites and males have five autosomes; the only difference being that the hermaphrodites have one pair X chromsomes while the male has one X chromosome.

Ecology

C. elegans lives in the soil across most of the temperate regions of the world.¹ In order to reproduce and grow it requires an humid environment, ambient temperature and atmospheric oxygen.¹ C. elegan feeds on diverse microorganism one such example would be bacteria.

In the laboratory it is easily maintained. Its food source in the lab would be e. coli. The C. elegans are grown on agar plates or in a liquid culture.

Application to Biotechnology

There are many advantages that allow it to be applied to biotechnology such as the rapid life cycle, ability to be easily cultivated in the laboratory, its small genome size, and the genetic analysis can be applied. Due to its usefulness in the lab it allows research to be done that answers variety of the problems in animal biology.

The C. elegans mutants allow a better understanding of the development and behavior of many animals. The genome of the C. elegans makes direct connections to many vertebrates. ¹ There is a degree of similarity between the genome of the human and the C. elegans; that allows a better understanding and improvement of the human biology. It is found that from 84 human disease genes that have been cloned 25 have direct orthologues in the C. elegans genome and a further 43 have a considerable similarity with nematode genes.¹ It also has significant impact on the medicine and agriculture fields by being a model organism for many studies of the parasitic nermatodes. The nervous system of the C. elegans is simple therefore allowing research opportunities for function and development in other animals.

References

1. Hope I.A, ed. C. elegans: A Practical Approach. New York: Oxford University Press, 1999.

2. Wood William Barry; ed. The Nematode Caenorhabditis Elegans. New York: Cold Spring Harbor Laboratory, 1988.

3. Riddle Donald, Blumenthal Thomas, Meyer Barbara, Priess James eds. C. elegans II. New York: Cold Spring Harbor Laboratory, 1997.