# Difference between revisions of "Dissociation constant"

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Given two molecules, '''A''' & '''B''', with initial [[molarity|molar]] concentrations '''[A]<sub>0</sub>''' and '''[B]<sub>0</sub>''', that form a reversible binding complex '''AB''', having a certain dissociation constant '''K<sub>d</sub>''', that is, | Given two molecules, '''A''' & '''B''', with initial [[molarity|molar]] concentrations '''[A]<sub>0</sub>''' and '''[B]<sub>0</sub>''', that form a reversible binding complex '''AB''', having a certain dissociation constant '''K<sub>d</sub>''', that is, | ||

− | :<math> \mathbf{A} + \mathbf{B} \ | + | :<math> \mathbf{A} + \mathbf{B} \leftrightarrows \mathbf{AB} </math> |

The Kd, by definition, is | The Kd, by definition, is | ||

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+ | |||

:<math> \mathbf{K_d} = \frac{\mathbf{[A]}\times\mathbf{[B]}}{\mathbf{[AB]}} </math> | :<math> \mathbf{K_d} = \frac{\mathbf{[A]}\times\mathbf{[B]}}{\mathbf{[AB]}} </math> |

## Latest revision as of 12:50, 22 June 2009

In biochemistry, chemistry and physics, the binding interaction of two molecules that bind with each other, for example a protein and a DNA duplex, is often quantified in terms of a **dissociation constant**, abbreviated as *K _{d}*, which is the inverse of the association constant, or K

_{a}. The strength of the binding interaction is inversely proportional to the K

_{d}. Extremely tight-binding molecules such as antibodies and the their target exhibit K

_{d}values in the picomolar range (10

^{-12}), while many drugs bind to their targets with K

_{d}values in the nanomolar (10

^{-9}) to micromolar (10

^{-6}) range. Given the Kd of an interaction, and the initial concentrations of the interacting molecules, the amount of complex can be calculated.

## Biomolecular Definition

Given two molecules, **A** & **B**, with initial molar concentrations **[A] _{0}** and

**[B]**, that form a reversible binding complex

_{0}**AB**, having a certain dissociation constant

**K**, that is,

_{d}

The Kd, by definition, is

Using the facts that and gives

expanding the top terms yields

Multiplying both sides by [AB] and rearranging gives a quadratic equation:

whose solution is:

Given the physical limitation that [AB] can not be greater than either [A]_{0} or [B]_{0} eliminates the solution in which the square root term is added to the first term.

## Implications

An inspection of the resulting solution shown above illustrates that in order to have an appreciable amount of bound material, the interacting molecules must be present at concentrations of 1/100 to 100 times the dissociation constant, as demonstrated in the table below, in which the concentrations of A and B are expressed in units of Kd.

[A]/Kd | [B]/Kd | %B bound ([AB]/[B])*100 |
---|---|---|

0.001 | 0.001 | 0% |

0.01 | 0.01 | 1% |

0.1 | 0.1 | 8% |

1.0 | 1.0 | 38% |

10 | 10 | 73% |

100 | 100 | 90% |

1000 | 1000 | 97% |