Melanocortins and appetite

= Melanocortins and appetite =

Overview of Pro-opiomelanocortin (POMC)
Pro-opiomelanocortin (POMC) is an important pro-hormone that gives rise to an array of bioactive peptide hormones that are implicated in energy balance, stress responses, pain, immune modulation, satiety, pigmentation and even exocrine gland secretions. It is expressed in the pituitary gland, skin, immune system and brain. In the brain it is expressed in the arcuate nucleus of the hypothalamus (ARH). POMC is post-translationally cleaved by a complex set of enzymes into a variety of hormones (see fig 1).

What is also interesting is that both B endorphin and α MSH are cleaved from the same prohormone (POMC) α MSH is known to reduce feeding behaviour as we'll see later but β endorphine is shown to increase appetite as endogenous B endorphin increases feeding behaviour (especially of highly palatable foods). It is therefore important to explore how the prohormone may be processed in different tissues and what dictates this. In the same sense, how are the cleavage enzymes regulated to produce various concentrations of the different peptide hormones?

Experiments in which the propeptide convertase enzymes are knocked out are not particularly helpful as PC1 and PC2 are expressed in cells other than POMC cells and have important physiological functions other than POMC cleavage. For example PC1 is essential for the biosynthesis of insulin and PC2 the biosynthesis of glucagon. However, it has been seen that PC2 KO mice exhibit no alpha MSH expression at all and Prader Willi patients have reduced hypothalamic PC2 expression. Millington et al 2003. The PC2 knock out mice are so defective in other ways(Scamuffa et al 2006) it is hard to tell whether the lack of alpha MSH has an effect. Prader Willi sufferers do exhibit an obese phenotype but again this syndrome comes as a result of a mutation of several genes so it cannot be inferred that PC2 mutation is solely responsible for the obese and hyperphagic phenotype.

Some evidence that leptin modifies POMC processing as leptin deficient mice show an upregulation of POMC and PC2 expression (Pritchard et al 2002). You cannot however infer from this that the proteins themselves are increased nor does it mean that alpha MSH production is increased. Further proteomic studies need to be done to work out a technique to measure αMSH in vivo.

Some recent experiments have shown that contrary to previous belief B endorphin may act in a complementary manner with alpha MSH in the long term as BEND-/- male mice are obese, thus suggesting that β endorphin has an unexpected anorexic phenotype (Appleyard et al 2003)

POMC was first noted for its importance in appetite and obesity in rodent studies. POMC-null mice were seen to exhibit hyperphagia and obesity without insulin resistance. Their adrenal gland was also atrophic and glucocorticoid levels were undetectable as a result of a lack of ACTH stimulating adrenal cell secretion and proliferation. This suggestion that POMC has a role in energy balance was supported by studies on rare individuals with POMC mutations. In humans a lack of POMC is fatal unless glucocorticoids are administered from birth as cortisol is essential for humans. A few rare individuals with the mutation have shown very similar phenotype to the POMC knockout mouse along with the characteristic red hair.

While these studies show that POMC-derived hormones may have a role in energy balance they don’t tell us which peptides are responsible for the effects and furthermore the lack of adrenal hormones as a secondary result of POMC lack may overshadow the primary POMC effects. Therefore we are required to look at the peptides in more detail. The main system implicated in energy balance is the melanocortin system.

Melanocyte-stimulating hormones and their Receptors
This is made up of several endogenous agonists such as α, β, γ MSH and interestingly has two endogenous inverse agonists as well namely the AgRP and agouti. These act on 5 different subtypes of the MCR (MCR1-5). See table1.

The Melanocortin Pathway
The control and regulation of feeding involves a complex interplay between circulating hormones, neurotransmitters, neuropeptides and nutrients, and the melanocortin system is a central component in this.

The POMC melanocortin system encompasses a number of CNS circuits including (1) POMC, AgRP and NPY which originate in the arcuate nucleus of the hypothalamus. (NPY is not a melanocortin, yet it acts on the Melancocrtin receptors to exert orexigenic effetcs). (2) POMC neurons that originate in the nucleus of the solitary tract (NTS) which (3) project to downstream targets of these POMC and AgRP neurons, acting on the POMC receptors, predominately MC3R and MC4R, to induce their effects on appetite regulation. AgRP, which acts in association with NPY was previously thought to be an antagonist of these receptors, yet it is now understood that it is an inverse agonist of these receptors.

In addition to its involvement in regulating energy homeostasis, the melanocortin system plays a role in mediating a number of physiological processes within the body including Cardiovascular control, inflamamtion, natriuresis and sexual function.

Inflammation Alpha –MSH also has potent anti-inflammatory effects although its precise mechanism is not fully understood, it is believed to influence vascular permeability and thus may act by decreasing it.

Cardiovascular Central application of γ- MSH (MC3R specific agonist, can induce an state of tachycardia, while the bradychardia that occurs following electrical stimulation of the arcuate nucleus can be inhibited by the administration of SHU-9119 into the DVC. As it acts through MC4R this highlights a potential role for this receptor in mediating cardiovascular control.

Natriuresis γ-MSH acts on MC3R in the kidney to promote natriuresis, and hence a deficiency of this peptide, or a mutation of the receptor has been shown to result in salt sensitive hypertension in mice.

Sexual Function Activation of MC4R plays a role in erectile function in males while MC3R is believed to induce a state of lordosis in females.

Although the melanocortin system is central to the regulatory mechanisms controlling appetite and satiety, the precise mechanism is not fully understood. Complexity arises from both the direct and indirect effects of a number of compounds including leptin, insulin, glucose, ghrelin, NPY, serotonin, peptide YY and endorphin, all of which act in isolation and in sync to mediate their effects on these POMC neurons.

Agonists	Antagonists Reduce food intake	Enhance food intake (hyperphagia) Increase energy expenditure	Decrease energy expenditure Reduce body weight	Increase body weight

This highlights the role of the melanocortin system in regulating energy homeostasis, and why disruption in the genes controlling this system, i.e. genetic mutations of the system, can result in individuals which are hyperphagic and consequentially obese.

A number of mutations of this system have been identified in mice, all of which show a dysregulation in energy homeostasis. Many of the mutations discovered involve excess production of POMC antagonists, so that melanocortin agonists can’t bind to melanocortin receptors to suppress appetite.

Obese individuals have presented with POMC deleterious gene mutations, as well as with heterozygous mutations in the MC4R receptor.
 * 1) Excess production of AgRP induces its antagonistic effects through binding to both the MC1R and MC4R.
 * 2) An increase in the expression of AgRP, which functions by antagonising receptors MC3R and MC4R. This prevents the potent appetite suppressor alpha MSH from binding.
 * 3) A mutation which results in a deficiency in the number of MC4R receptors.
 * 4) The insufficient production of POMC derived peptides to bind to these receptors.

POMC neurons and their peptides mediate satiety signals, while NPY neurons induce hunger signals and decrease energy expenditure. This integration involves both long term signals (leptin from adipose tissue and insulin), as well as acute hunger/satiety signals from the brainstem.

The melancortin alpha MSH is one of the most important regulators of energy homeostasis in the hypothalamus, where it induces a state of satiety within an individual through its actions on the MC4R. Additionally, administration of ACTH into certain regions of the hypothalamus has similar effects. Agouti is an antagonist at MC1R and MC4R receptors, while AgRP incurs antagonistic effects through its action on MC3R and MC4R receptors. Due to suppression of the alpha MSH anorectic signal, mutant mice with ectopic expression of these peptides are hyperphagic with an increase in adipose mass, lean mass, hyperinsulinemia and consequentially are obese.

Melanocortins are peptides derived form POMC, and are determined by tissue-specific post-translational cleavage. POMC gene expression has been identified in a number of tissues including the pituitary, skin, immune system and hypothalamus, which highlights the many physiological processes that POMC is involved in. POMC and CART are co-expression in a subpopulation of neurones in the arcuate nucleus, and the expression of both is stimulated by leptin. AgRP and alpha-MSH are involved in appetite regulation via their actions on MC4R. Melanocortin receptors MC3R and MC4R are unique in the sense that have both endogenous agonists and antagonists. Obesity can arise as a result of a POMC deficiency. Some melancortin receptors have been found on adipocytes (MC4R), suggesting that peripheral detection of these circulating melanocortins may also be involved in regulating energy homeostasis. Expression of these peptides in the anterior pitiutuary, results in the production of ACTH via the cleavage of POMC by the enzyme prohormone convertase1 (PC1). Stimulation of adrenal steroiogenesis then occurs via the action of ACTH on MC2R.

Alpha-MSH Alpha- MSH, has been widely accepted as the main endogenous agonist, derived from the POMC gene, which is cleaved in the hypothalamus. It exerts its anorexigenic effects through MC3R and MC4R. In addition to suppressing appetite, alpha- MSH is has been shown to increase both metabolism and body temperature, which is referred to a coordinated catabolic pattern. Balasko et.al 2010 administered i.c.v alpha-MSH to rats and found that in addition to a reduction in food intake, the rats also had an increased metabolic rate.

Leptin is produced primarily in white adipose tissue but also to some extent in brown adipose tissue, stomach, placenta, while recent studies suggest that it may also be produced in the brain. Numerous leptin receptors have been identified, all of which transmit their signal via the JAK-STAT pathway. The leptin receptor involved in regulating appetite is the receptor type Ob-Rb, which is present in many brain regions including ARC, PVN, DMH and LHA of the hypothalamus. When leptin binds to the Ob-Rb receptor, the subsequent signalling cascade results in a decrease in appetite through inhibition of the peptides involved in inducing orexigenic effects (NPY, MCY, AGRP and orexins). Additionally the anorexigenic peptides peptides (alpha-MSH, CART and CRH) are stimulated. Excess body weight results in excess leptin production from adipose tissue(released in a regular pulsatile manner) and hence leptin can act to restore normal energy homeostasis, by suppressing appetite and increasing energy expenditure through its effect on anorextic peptides.

Leptin secreted form adipose tissue and insulin secreted from the pancreas, also regulate food intake. Excess adipose tissue (as occurs in obese individuals) results in an increase in leptin production (which normally induces a feeling of satiety), but excess production of NPY (as occurs in some mutations of the melancortin system), can suppress its effects. Similarly insulin levels show a marked increase in obese individuals, with hyperinsuliemia being one of the first metabolic disturbances identified in those obese subjects with mutations of the melanocortin system.

Insulin Following meal ingestion, there is a dramatic increase in insulin levels, some of which crosses the blood brain barrier in a concentration that is representative of circulating insulin levels. Central administration of insulin has been shown to act on both NPY and POMC systems, and increases POMC mRNA synthesis as well inhibiting food intake in fasting rats. Rats with untreated diabetes have shown to have a diminished amount of POMC mRNA which is indicative of its involvement in stimulating its synthesis.

Ghrelin Ghrelin stimulates arcuate nucleus production of NPY and AgRP, while simultaneously inhibiting the suppressive effects that leptin induces on appetite. Ghrelin administration in rats produces hyperphagia and increased body weight, proportional to the amount of ghrelin given. The role of ghrelin within the melanocortin system has been verified, whereby central administration of this peptide results in excessive eating, but if NPY antagonists are given in conjunction,a state of hyperphagia is not induced. During periods of meal deprivation, ghrelin levels increase. They induce their potent appetite stimulating properties by activating arcuate NPY and AgRP expression. In contrast, following food consumption, ghrelin levels show a marked decrease.

An example of the complexity that arises in fully understanding the precise mechanism governing this system arises from mice AgRP KO models. Interestingly, while the involvement of AgRP in the melanocortin system is undisputed, one would expect such KO to present with altered phenotypes and eating patterns, yet these AgRP were just like their wild type counterparts. NPY has been identified as one of the most abundant and potent orexigenic peptides of the hypothalamus. While ghrelin ahs short term effects, administration of NPY produces a prolonged and substantial increase in food consumption and a decrease in thermogenesis, resulting in an overall increase in body weight and leading to obesity. NPY exerts its action via G- protein coupled receptors, 5 of which have been identified, of which Y5 has been identified as being involved in meidatign its effect on feeding. Circulating levels of leptin and insulin can regulate NPY synthesis, while glucocorticoids serve to stimulate it. During times of fasting, low levels of insulin and leptin result in the up-regulation of NPY synthesis. Interestingly, an obvious increase in ARC NPY neuronal activity is not recorded in diet induced obese individuals, which suggests that it may be involved in ensuring a certain amount of energy stores, but when this store is in excess, NPY neurons exhibit diminished activity, potentially in an effort to restore normal energy homeostasis.

AgRP- inhibits the effects of alpha- MSH on inducing its anorexigeneic effect. AGRP is potent in activating appetite through its anatagonistic activity on both MC3R and MC4R and is expressed only in the arcuate nucleus of the hypothalamus All neurons expressing AgRP also produce NPY, and project to various brain sites including PVN and DMH. Leptin inhibits the expression of AgRP, while periods of starvation result in an increase in its expession. Unlike NPY which induces strong, short lived effects on stimulating appetite, AgRP administration can induce a state of hyperphagia for up to one week.

Thus, when energy stores are low, there is reduced leptin released from adipose tissue, which in turn allows in an increase in the production of orexigenic peptides including NPY, AgRP and a decrease in alpha-MSH and CART. The opposite is true in times if positive energy imbalance.

Although Peptide YY is an appetite suppressor, it appears to mediate its effects through a distinct pathway that does not involve the melanocortin system as both POMC and MC4R KO mice continued to show a decrease in food intake following its administration, indicating that another system may be involved. This further highlights the complexity of the mechanisms controlling energy homeostasis.

Suggested future studies
Despite the unquestionable importance of melanocortins within appetite regulation much of the underlying molecular mechanisms that facilitate the melanocortin signalling pathways are still under debate and many aspects remain unidentified.

Melanocortin Receptors G-proteins: A question of cross-talk? POMC expression is up-regulated in αMSH neurones as result of increased leptin and insulin signalling. This has been proposed to sequentially increase αMSH release which acts predominantly through activation of the G-protein coupled receptors MC3R and MC4R which are Gs linked receptors. Gs stimulate the up-regulation of adenylase cyclase and thus increase cAMP levels, and a multidude of sequential kinase signalling resulting in a complex cascade of second messengers that facilitate its anorexigenic effects and prevent orexigenic pathways.

Recent studies have unveiled the possibility for the involvement of alternative G-proteins or the coupling of G-proteins to create a specific effect. However, there are contradictory studies between the involvement of Gq’s and the activation of PLC and calcium signal which has been seen in some studies but not in others however, the cell lines used were different and furthermore the results are yet to be confirmed in vivo. Additionally, another study found that the inhibition of Gi/o specific cells expressing MC4R treated with agonist created a suppression of ERK1/2 phosphorylation indicating a possible role for Gi’s and downstream PKC/calcium activation of ERK 1/2 as opposed to conventional Gs PKA signalling initially described in certain cell types. Clearly, these areas of research is open to clarification in terms of the G-proteins involved in melanocortin signalling and activation of important downstream activity of molecules such as ERK 1/2 and thus enable us to fine tune the understanding of appetite regulation.

αMSH: Please release me let me go Although, as we mention above it is thought that leptin and insulin are actively responsible for increasing POMC expression and consequently αMSH release very little is understood about how and where αMSH is actually released.

Melanocortin 3 Receptor Although there are implications for the MC3R in appetite regulation it does not have such in-depth association with obesity as the MC4R has. Needless to say its involvement should not be overlooked especially since studies have shown that mutations, heterozygous genotype and knock out experiments can create the pathophysiological state or at least predispose humans and murine species to obesity either in childhood or later in life which is especially apparent when coupled to an obesigenic environment. Moreover, there is confusion as to the roles that this receptor has in an anorexic effect since it is also thought that it is part of the auto-regulation of POMC providing negative feedback. Especially since it seems that from studies by A. A Butler et al. 2000 that MC3R knock out shows primarily alterations to in metabolic syndromes whereas MC4R alters feeding behaviour and intake as well as energy expenditure. The distinction between MC3Rs actions in terms of obesity need further elucidation such that in future it may be required in combined therapeutic techniques to target the melanocortin receptors.

Agouti-related protein signalling: antagonist or inverse agonist? AgRP is thought to have a competitive antagonistic role on MCR to prevent αMSH signalling however, research has unveiled that AgRP can produce autonomous orexigenic effects by reducing the cAMP increase caused by binding of αMSH potentially through Gi signalling suggesting that it has inverse agonist qualities. This is primarily observed in terms of its ability to reduce cAMP levels as shown by researchers such as W A. J. Nijenhuis and colleagues Moreover, recent studies have also reiterated these findings for a AgRP's role as an inverse agonist in-vivo as well as eluding to recent studies in MC4R knock out mice in which AgRP induced hyperphagic state was still observed suggestive of its action through a receptor independent of the melanocortin receptors. The exact composition of MCRs and the complexes that are formed at the membrane is an avenue that requires exploration especially since the picture is far more complex than you might think as MCR could potentially interact with a variety of G-proteins, accessory proteins such as syndecans, mahoganoid and especially MRAPs with MC4R in order to manipulate various aspects of αMSHs signalling capacity.

Lipid rafts The cholesterol composition of the lipid membrane affects cAMP signalling, with higher levels of cholesterol dampening the cAMP response because the lipid rafts segregate the G-protein signalling components accessibility to each other. Accordingly, this reveals that the lipid micro-domain surrounding the MCR’s location within the membrane may alter the result of αMSH signalling that possibly produces cell-specific effects. However, this is also a vague area in terms of solid evidence and requires studies across different cell lines and into cell membrane composition specific to the MCR location which could potentially be quite challenging .

A role for BNDF/Trk signalling The neurotrophic brain derived neuronal growth factor (BNDF) acts through the tropomysin receptor kinase B (Trk) and is thought to be activated downstream of melanocortin signalling through MC4R. This is proposed to be due to the ability of synthetic mineralocorticoid peptides to promote BDNF expression within hypothalamic nuclei, the ventromedial hypothalamus (VMH), in addition to the ability of BDNF to blunt the feeding in MC4R knockout mice. However, the manner which they interact is not clear and further investigation at the molecular level will shine light on the downstream signalling involved through protein-protein interaction assay techniques such as a GST protein interaction pull down of the 'bait' protein, possibly the section of protein thought most likely to interact with another protein, and wash with cell lysates containing potential 'prey' molecule candidates followed by immunoblotting to assess interactions

Thyroid hormone

As well as being expressed in a variety of other neuronal cell types the MC4R has been found on hypothalamic paraventricular Thyrotropin-releasing hormone (TRH) neurones. This is hardly surprising due to thyroid hormones essential role in metabolism and thus energy homeostasis. Studies by Stéphanie Decherf in 2009 identify that inhibitor feedback from thyroxins (T4) biologically active derivative triodothyronine (T3)is occurring at the MC4R .This incidentally alleviates inhibition of food intake and allows the activation of orexigenic pathways. The negative feedback was seen to be strongest in the brainstem region where it is suggested to regulate meal size and thermogenesis. The participation of yet another regulatory pathway adds to this multi-factorial system that regulates metabolism and energy homeostasis and highlights the reasons why obesity is such a complex disease to tackle. The role of T3s action on MC4R is an interesting angle and would benefit from assessing where and how T3 is acting on MC4R in other neuronal populations within the CNS. Moreover, would it ever be a sensible area to feasibly target in the future, pharmacologically?

Corticotropin Releasing Hormone (CRH) CRH has also been discovered to partake in appetite regulation and fairly recent studies using double labelling in situ hybridization of CRH neruons has found that MC4R is also located on these neurones of the PVN. Several studies show that CRH is one of the elements regulated downstream of MC4R activation and aids arexogenic systems. Consequently, linking the melanocortin pathway to the hypothalamic pituitary axis and thus to an involvement in stress. X. Y Lu ''et. al'' (2003) proposes the implications of this connection to stress as resulting in a relation to eating disorders and therefore is of particular interest not merely in treating anorexia nervosa but in terms of potentially opening an avenue of insight into obesityies polar opposite and help aid the research into the requirements for the 'happy medium'. However, more work is required in terms of the exact receptor type that CRH is acting on and also whether the HPA axis requires melanocortin signalling or likewise is the HPA axis required for appropriate melanocortin regulation of feeding?

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